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We sreened extracts against a ß-galactosidase-expressing, transgenic Trypanosoma cruzi (Tulahuen C4 strain). Intracellular forms, amastigotes and trypomastigotes, cause disease in humans and therefore are the likely target for medication. In the bioassay T. cruzi are grown intracellularly in Vero cells. For both the intracellular assay and extracellular assay (with axenic epimastigotes), cells are cultured with 50 and 10 microg mL-1 of extract for three days. Nifurtimox at and amphotericin are used as a positive controls. T. cruzi growth is determined from the cleavage of Chlorophenol red-ß-D-Galactopyranoside (CPRG) and the color is read at 570nm. 
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Samples are tested for activity in bioassays against cancer, HIV,leishmaniasis, Chagas' disease, malaria and agricultural
pests. |